Key features and details | |
Cat. No. | MABL-2630 |
Name | Anti-PCT mAbs |
Clone No. | AFD- PCT Nb3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | SPR, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for PCT. PCT is a protein consisting of 116 amino acids. PCT is the peptide precursor of calcitonin, a hormone that is synthesized by the parafollicular C cells of the thyroid and involved in calcium homeostasis. |
Alternative Name | Procalcitonin |
UniProt | |
Immunogen | The original antibody was isolated by panning a camel-derived phage display nanobody library against PCT. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis (CN104404630A). The binding affinity of the VHH fragment to PCT was measured by surface plasmon resonance (Kd= 8.9 nM). The original format of the antibody was successfully used as a detection antibody, together with anti-PCT Nb2, for the development of a sandwich ELISA for the detection of PCT (Yan et al., 2015; PMID: 25944262). |
Antibody First Published | Yan et al. Characterization and applications of Nanobodies against human procalcitonin selected from a novel naïve Nanobody phage display library J Nanobiotechnology. 2015 May 6;13:33. doi: 10.1186/s12951-015-0091-7. PMID:25944262 |
Note on publication | The paper describes the generation and characterization of the antibody by phage display selection. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
