Anti-PD-1 mAbs

Cat. No.

MABL-2635

Name

Anti-PD-1 mAbs

Clone No.

AFD- 6#

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, inhibition, SPR, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for PD-1. PD-1 is a type I membrane protein with 288 amino acids, and is one of the main known  immune checkpoints. PD-1 is expressed in activated T lymphocytes, and it   binds ligands PD-L1 and PD-L2.

Alternative Name

CD279; Programmed cell death protein 1;   PDCD1; PD-1; hPD-1; Human programmed cell death receptor-1

UniProt

Q15116

Immunogen

The original antibody was generated by   immunizing BALB/c mice with PD-1.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. The original format and the   humanized version of the antibody were capable of blocking the binding of   PD-1 and PD-L1. The antibody was able to activate the T cells under in vitro   culture conditions and promote the production of IFN-γ, thereby enhancing   their T cell immune function. The binding affinity of the murine and   humanized versions of the antibody to PD-1 were measured by surface plasmon   resonance (Kd= 6.74×10 - 10 M and 8.07×10 -10 M respectively). The antibody   blocked the interactions between PD-1 and PD-L1. The antibody inhibited tumor   growth in NCG mouse models of non-small cell lung cancer and colorectal   cancer tumors (CN107043420B).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.