Anti-PD-1 mAbs

Cat. No.

MABL-2638

Name

Anti-PD-1 mAbs

Clone No.

AFD- J116

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human PD-1.

Alternative Name

CD279; PD1; PD 1; Programmed cell death   protein 1; Programmed cell death 1; pdcd1; Protein PD-1; hPD-1

UniProt

Q15116

Immunogen

The original antibody was raised by   immunising BALB/c mice with a human PD-1:IgG1 fusion protein.

Application Notes

In immunohistochemical analysis, this antibody has been used to stain for PD-1 expression in human breast cancer   frozen tissue sections (Ghebeh et al, 2008), human tonsil cryosections (Iwai   et al, 2002), acetone-fixed liver tissue sections (Wang et al, 2011) and   formalin-fixed, paraffin- embedded human gastric neuroendocrine carcinoma   tissue sections (Yang et al, 2019). This PD-1 blocking antibody has been   used, in combination with an anti-PD-L1 antibody, to assess the effects of   PD-1 pathway blockade on tumor-infiltrating lymphocytes (Matsuzaki et al,   2010). It has also been used to block PD-1 in samples of human peripheral   blood and pleural fluid mononuclear cells (Jurado et al, 2008), and to test   the effects of PD-1 blockade on peripheral blood mononuclear cell   proliferation and interferon‐γ production in samples from HIV-1-positive   patients (Rosignoli et al, 2009).

Antibody First   Published

Iwai et al. Microanatomical localization of PD-1 in human tonsils Immunol Lett. 2002 Oct 1;83(3):215-20 PMID:12095712

Note on publication

Describes the original generation and   characterisation of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.