Key features and details | |
Cat. No. | MABL-2638 |
Name | Anti-PD-1 mAbs |
Clone No. | AFD- J116 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human PD-1. |
Alternative Name | CD279; PD1; PD 1; Programmed cell death protein 1; Programmed cell death 1; pdcd1; Protein PD-1; hPD-1 |
UniProt | Q15116 |
Immunogen | The original antibody was raised by immunising BALB/c mice with a human PD-1:IgG1 fusion protein. |
Application Notes | In immunohistochemical analysis, this antibody has been used to stain for PD-1 expression in human breast cancer frozen tissue sections (Ghebeh et al, 2008), human tonsil cryosections (Iwai et al, 2002), acetone-fixed liver tissue sections (Wang et al, 2011) and formalin-fixed, paraffin- embedded human gastric neuroendocrine carcinoma tissue sections (Yang et al, 2019). This PD-1 blocking antibody has been used, in combination with an anti-PD-L1 antibody, to assess the effects of PD-1 pathway blockade on tumor-infiltrating lymphocytes (Matsuzaki et al, 2010). It has also been used to block PD-1 in samples of human peripheral blood and pleural fluid mononuclear cells (Jurado et al, 2008), and to test the effects of PD-1 blockade on peripheral blood mononuclear cell proliferation and interferon‐γ production in samples from HIV-1-positive patients (Rosignoli et al, 2009). |
Antibody First Published | Iwai et al. Microanatomical localization of PD-1 in human tonsils Immunol Lett. 2002 Oct 1;83(3):215-20 PMID:12095712 |
Note on publication | Describes the original generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
