Key features and details | |
Cat. No. | MABL-2639 |
Name | Anti-PD-1 mAbs |
Clone No. | AFD- J43 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | depleting, IP, neutralising, ELISA, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody is specific for murine PD-1. |
Alternative Name | CD279; PD1; PD 1; Programmed cell death protein 1; Programmed cell death 1; pdcd1; Protein PD-1; mPD-1 |
UniProt | Q02242 |
Immunogen | This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells. |
Application Notes | The specificity of this antibody has been confirmed in ELISA analysis, using PD-1 extracellular domain fusion proteins (Agata et al, 1996). Additionally, in flow cytometric analysis, this antibody reacts with PD-1 cDNA-transfected BHK and CHO cells, but not with parental BHK and CHO cells, as well as reacting with lymphocytes from PD-1 cDNA transgenic mice (Agata et al, 1996). This antibody has been used to immunoprecipitate PD-1 from lysates of PD-1 cDNA-transfected BHK and CHO cells (Agata et al, 1996), in flow cytometric quantification of CD4+PD-1+ T cells in murine spleens (Kasagi et al, 2010), and in immunohistochemical analysis of acetone-fixed murine spinal cord and brain tissue sections (Salama et al, 2003). This antibody displays diverse effects in different mouse models of disease. When administered to NZB/W F1 mice, a model of lupus-like nephritis, this antibody has been shown to delay the onset of nephritis and prolong survival, through the depletion of PD-1+ T cells (Kasagi et al, 2010). |
Antibody First Published | Agata et al. Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes. Int Immunol. 1996 May;8(5):765-72. PMID:8671665 |
Note on publication | Describes the original generation of this antibody, and its use in ELISA, flow cytometry and immunoprecipitation analyses |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
