Key features and details | |
Cat. No. | MABL-2640 |
Name | Anti-PD-1 mAbs |
Clone No. | AFD- JC053 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, WB, IHC, ELISA |
Species Reactivity | Dog |
Basic Information | |
Specificity | This antibody binds canine PD-1. |
Alternative Name | CD279; Programmed death 1 receptor; PD1; PD 1 |
UniProt | A0A024FCJ9 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with recombinant canine PD-1 using an exponentially increasing dosing strategy with MPLA as adjuvant. |
Application Notes | This antibody is recommended for detection of canine PD-1. This antibody can detect canine PD-1 in a western blot assay. JC053 successfully detected canine PD-1 expressed on formalin-fixed paraffin-embedded (FFPE) tonsil and lymph node via immunohistochemistry suggesting that this could be useful for histological detection of canine PD-1 and as diagnostic tool in tumor biopsies. JC053 was also used to determine the PD-1 expression on CD4+ and CD8+ T-cells of canine PBMCs stimulated with ConA using flow cytometry. It could also detect recombinant canine PD-1 expression on CHO and CLGL-90 cells in flow cytometry. The ability of the antibody to block interactions between PD-1 and PD-L1 was checked using ELISA. It is known that blockade of PD-L1 and PD-1 interactions causes increase in Ifn-γ level. This antibody did not effectively block PD-1 and did not cause a recovery of T cell function (PMID: 32614928). |
Antibody First Published | Choi et al. Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function. PLoS One. (2020); 15(7): e0235518 PMID:32614928 |
Note on publication | Describes the generation and characterization of antibodies towards canine PD-1 and PD-L1 for their potential use in therapeutics for veterinary oncology. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
