Key features and details | |
Cat. No. | MABL-2642 |
Name | Anti-PD-1 mAbs |
Clone No. | AFD- RMP1-14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, FC, IHC-Fr |
Species Reactivity | Mouse |
Basic Information | |
Specificity | This antibody binds to murine PD-1. |
Alternative Name | CD279; PD1; PDCD1; RMPI-14; RMP1/14; Programmed Death Receptor 1; Programmed Death 1 |
UniProt | |
Immunogen | The original rat hybridoma was generated by immunizing Sprague Dawley rats with mouse PD-1-transfectked BHK cells. A P3U1 myeloma was used as the fusion partner. |
Application Notes | Binding of the original rat IgG2a antibody to PD1 has been reported to prevent binding of both B7-H1-Ig (PD-L1-Ig) and B7-DC-Ig (PD-L2-Ig) fusion proteins to PD1. |
Antibody First Published | Yamazaki et al. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 2005 Aug 1;175(3):1586-92 PMID:16034097 |
Note on publication | Describes the generation and initial characterisation of the RMP1-14 hybridoma |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
