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Anti-Pf12p mAbs

简要描述:

The antibody recognizes Pf12p. The antibody does not cross-react with Pf12 or Pf41. The antibody binds specifically to the Pf12p interdomain (D1-D2 junction) area.

产品参数:

Cat. No.:MABL-2676

Species:Engineer

Species Reactivity:Plasmodium falciparum

Type:Recombinate

Application:crystallization, SPR, WB, ELISA

详细介绍

Key features and   details


Cat. No.

MABL-2676

Name

Anti-Pf12p mAbs

Clone No.

AFD- B9

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, SPR, WB, ELISA

Species Reactivity

Plasmodium falciparum

Basic Information


Specificity

The antibody recognizes Pf12p. The antibody   does not cross-react with Pf12 or Pf41. The antibody binds specifically to   the Pf12p interdomain (D1-D2 junction) area.

Alternative Name

Surface protein P12p

UniProt

C6KSX1

Immunogen

The original antibody was generated by   immunizing an alpaca with recombinant Pf12p protein and constructing a phage   library. The antibody was isolated by biopanning against Pf12p.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. The antibody recognised reduced and non-reduced Pf12p in western blot analysis. The binding affinity of the   original format of the antibody to Pf12p was measured by surface plasmon   resonance (Kd= 6.7 nM). The structure of the antibody in complex with   recombinant Pf12p was determined by X-ray crystallography (Dietrich et al.,   2021; PMID: 33480416).

Antibody First   Published

Dietrich et al. Nanobody generation and   structuralcharacterization of Plasmodium falciparum6-cysteine protein Pf12p   Biochem J. 2021 Feb 12;478(3):579-595. doi: 10.1042/BCJ20200415.   PMID:33480416

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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