Key features and details | |
Cat. No. | MABL-2676 |
Name | Anti-Pf12p mAbs |
Clone No. | AFD- B9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, SPR, WB, ELISA |
Species Reactivity | Plasmodium falciparum |
Basic Information | |
Specificity | The antibody recognizes Pf12p. The antibody does not cross-react with Pf12 or Pf41. The antibody binds specifically to the Pf12p interdomain (D1-D2 junction) area. |
Alternative Name | Surface protein P12p |
UniProt | C6KSX1 |
Immunogen | The original antibody was generated by immunizing an alpaca with recombinant Pf12p protein and constructing a phage library. The antibody was isolated by biopanning against Pf12p. |
Application Notes | The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody recognised reduced and non-reduced Pf12p in western blot analysis. The binding affinity of the original format of the antibody to Pf12p was measured by surface plasmon resonance (Kd= 6.7 nM). The structure of the antibody in complex with recombinant Pf12p was determined by X-ray crystallography (Dietrich et al., 2021; PMID: 33480416). |
Antibody First Published | Dietrich et al. Nanobody generation and structuralcharacterization of Plasmodium falciparum6-cysteine protein Pf12p Biochem J. 2021 Feb 12;478(3):579-595. doi: 10.1042/BCJ20200415. PMID:33480416 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |