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Anti-PfMSP-1 mAbs

简要描述:

This antibody specifically recognises a disulphide-dependent conformational epitope of the native Plasmodium falciparum merezoite surface protein 1.

产品参数:

Cat. No.:MABL-2677

Species:Engineer

Species Reactivity:Plasmodium falciparum

Type:Recombinate

Application:​immunoblot, IP, ELISA, IF

详细介绍

Key features and   details


Cat. No.

MABL-2677

Name

Anti-PfMSP-1 mAbs

Clone No.

AFD-5.2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

immunoblot, IP, ELISA, IF

Species Reactivity

Plasmodium falciparum

Basic Information


Specificity

This antibody specifically recognises a   disulphide-dependent conformational epitope of the native Plasmodium   falciparum merezoite surface protein 1.

Alternative Name

PMMSA; P. falciparum merozoite surface   protein 1; MSP-1; p195

UniProt

P04933

Immunogen

This antibody was raised by immunising BALB/c   mice with schizont-enriched P. falciparum.

Application Notes

This antibody has been used in indirect   immunofluorescence analysis to stain schizonts and free merezoites in   acetone-fixed parasite samples (Siddiqui et al, 1986), and in   immunoprecipitation (Siddiqui et al, 1986) and ELISA (Yoshida et al, 1999;   Mazumdar et al, 2009) experiments. This antibody reacts strongly with native   PfMSP-1 in immunoblot and ELISA analysis, but reactivity declines under   denatured conditions ( Mazumdar et al, 2009).

Antibody First   Published

Siddiqui et al. Induction of Protective   Immunity to Monoclonal-Antibody- Defined Plasmodium falciparum Antigens   Requires Strong Adjuvant in Aotus Monkeys Infect Immun. 1986 Apr;52(1):314-8.   PMID:3514459

Note on publication

Describes the original generation of this   antibody, and its characterisation in IHC and IP analysis.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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