Key features and details | |
Cat. No. | MABL-2677 |
Name | Anti-PfMSP-1 mAbs |
Clone No. | AFD-5.2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunoblot, IP, ELISA, IF |
Species Reactivity | Plasmodium falciparum |
Basic Information | |
Specificity | This antibody specifically recognises a disulphide-dependent conformational epitope of the native Plasmodium falciparum merezoite surface protein 1. |
Alternative Name | PMMSA; P. falciparum merozoite surface protein 1; MSP-1; p195 |
UniProt | P04933 |
Immunogen | This antibody was raised by immunising BALB/c mice with schizont-enriched P. falciparum. |
Application Notes | This antibody has been used in indirect immunofluorescence analysis to stain schizonts and free merezoites in acetone-fixed parasite samples (Siddiqui et al, 1986), and in immunoprecipitation (Siddiqui et al, 1986) and ELISA (Yoshida et al, 1999; Mazumdar et al, 2009) experiments. This antibody reacts strongly with native PfMSP-1 in immunoblot and ELISA analysis, but reactivity declines under denatured conditions ( Mazumdar et al, 2009). |
Antibody First Published | Siddiqui et al. Induction of Protective Immunity to Monoclonal-Antibody- Defined Plasmodium falciparum Antigens Requires Strong Adjuvant in Aotus Monkeys Infect Immun. 1986 Apr;52(1):314-8. PMID:3514459 |
Note on publication | Describes the original generation of this antibody, and its characterisation in IHC and IP analysis. |
COA Information (For reference only, actual COA shall prevail) | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |