Key features and details | |
Cat. No. | MABL-2693 |
Name | Anti-Phosphotyrosine mAbs |
Clone No. | AFD- PY20 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IHC-FoFr, IHC-P, IP, WB, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | PY20 recognizes phospho-tyrosine and phosphotyrosine containing proteins with a reported Kd of 500 nM for the original clone.Whilst phospho-Phenylalanine (Kd = 4 uM M), phospho-Serine (>100 mM) & phospho-Threonine (100 mM) are also detected, the differences in affinity make this antibody primarily phospho-tyrosine specific. |
Alternative Name | p-Tyr; Phospho-tyrosine; p-Y |
UniProt | |
Immunogen | Phosphotyrosine conjugated to Keyhole limpet hemocyanin (KLH). |
Application Notes | Protein phosphorylation is a key mechanism of signal transduction in Eukaryotes and Prokaryotes. PY20 has been a crucial antibody in the investigation of these processes. An excellent comparison of anti-phosphotyrosine antibodies can be found here . |
Antibody First Published | Glenney et al. Monoclonal antibodies to phosphotyrosine. J Immunol Methods. PMID:2452204 |
Note on publication | Describes generation of anti-phosphotyrosine antibodies, including PY20 |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
