Anti-Phosphovimentin mAbs

Cat. No.

MABL-2694

Name

Anti-Phosphovimentin  mAbs

Clone No.

AFD- 4A4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, WB, IF

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody specifically binds mouse and   human vimentin phosphorylated at serine 55 by cdc2 kinase . This antibody is incapable of binding non-phosphorylated vimentin or with vimentin   phosphorylated by other kinases.

Alternative Name

MPV55; HPV55; phosphorylated vimentin;   vimentin phosphoSer55

UniProt

P20152

Immunogen

The original antibody was generated by   immunizing BDF1 mice intraperitoneally with a MPV55-KLH emulsified in   complete Freund's adjuvant. MPV55-KLH is a mouse vimentin phosphopeptide   MPV55 (Ser-Leu-Tyr-Ser-Ser-phosphoSef15-ProGly-Gly-Ala-Tyr-Cys) which was   coupled to keyhole limpet hemocyanin (KLH) with   m-maleimido-benzoyl-N-hydroxysuccinimide ester.

Application Notes

This antibody was developed to investigate   the role of intermediate filament (IF) protein phosphorylation by cdc2 kinase   during mitosis. The binding specificity of 4A4 towards serine 55   phosphorylated vimentin was determined by ELISA. Western blotting indicated   that this antibody reacted with vimentin phosphorylated by cdc2 kinase but   not with non-phosphorylated vimentin or with vimentin phosphorylated by other   kinases such as CAMP-dependent protein kinase, protein kinase C, or Ca2+-   calmodulin-dependent protein kinase 11. Immunofluorescence and immunoelectron   microscopy showed that vimentin Ser55 residues distributed in the entire   cytoplasmic vimentin filament system are phosphorylated when the cells enter   mitosis and dephosphorylated in cytokinesis (PMID: 7983050). This antibody   was also used in the visualization of mitotic radial glial lineage cells in   the developing rat brain which revealed different patterns of gliogenesis in   the neocortex and cerebellum (PMID: 9633804). 

Antibody First   Published

Tsujimura et al. Visualization and function   of vimentin phosphorylation by cdc2 kinase during mitosis. J Biol Chem.   (1994); 269(49):31097-106. PMID:7983050

Note on publication

Decribes the generation of this antibody to   determine the role of phosphorylation by cdc2 kinase during mitosis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.