Anti-spike protein mAbs

Cat. No.

MABL-2967

Name

Anti-spike protein mAbs

Clone No.

AFD- PZZ 21

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo, neutralizing, ELISA, IF

Species Reactivity

Porcine epidemic diarrhea virus

Basic Information

Specificity

The antibody binds the S1 region of the   spike protein of PEDV. The S protein is a type I glycoprotein that plays a   crucial role in virus attachment, entry, receptor binding, cell membrane   fusion and induction of neutralizing antibodies. The S protein can be cleaved   into S1 (residues 1–789) and S2 subunits (residues 790–1386) by host   protease. The S1 subunit contains the N-terminal domain (NTD, residues 1–233)   that shows sialic acid binding activity and the C-terminal domain (CTD, residues 253–638) that attaches to the cell surface receptor (e.g.,   aminopeptidase N (APN)). The S2 subunit mediates virus–cell membrane fusion.

Alternative Name

Spike glycoprotein; S glycoprotein; E2; Peplomer protein

UniProt

Q91AV1

Immunogen

A scFv antibody phage display library was constructed   from peripheral blood lymphocytes of piglets induced with PEDV. The library was screened with four rounds of biopanning using purified PEDV antigen, and   scFv antibodies that bound to PEDV were obtained.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. Immunofluorescence assay   revealed that the scFv stained PEDV-infected Vero E6 cells but did not react   with uninfected control cells. The scFv was shown to inhibit PEDV infectivity   by the plaque reduction neutralization assay (the virus neutralization titer   was 6.25 µg/mL). Results showed the a cocktail of the scF fragment, PZZ 24   and PZZ 35 neutralized viral infection at 3.125 µg/mL. Finally, piglets   orally administered with a mixture of scFv fragment, PZZ 24 and PZZ 35 showed   no to mild clinical symptoms, significantly less viral shedding, no mortality   and no intestinal lesions.

Antibody First   Published

Zhang et al. Single Chain Fragment Variable (scFv) Antibodies Targeting the Spike Protein of Porcine Epidemic Diarrhea   Virus Provide Protection against Viral Infection in Piglets. Viruses.2019 Jan; 11(1): 58. PMID:30646521

Note on publication

 The original paper describes the generation   and characterization of the antibody

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.