Anti-spike protein mAbs

Cat. No.

MABL-2968

Name

Anti-spike protein mAbs

Clone No.

AFD- PZZ 24

From

Recombinant Antibody

Isotype

Engineer antibody

Application

in vivo protection, ELISA, IF

Species Reactivity

Porcine epidemic diarrhea virus

Basic Information

Specificity

The antibody binds the S1 region of the   spike protein of PEDV. The S protein is a type I glycoprotein that plays a   crucial role in virus attachment, entry, receptor binding, cell membrane   fusion and induction of neutralizing antibodies. The S protein can be cleaved   into S1 (residues 1–789) and S2 subunits (residues 790–1386) by host   protease. The S1 subunit contains the N-terminal domain (NTD, residues 1–233)   that shows sialic acid binding activity and the C-terminal domain (CTD,   residues 253–638) that attaches to the cell surface receptor (e.g.,   aminopeptidase N (APN)). The S2 subunit mediates virus–cell membrane fusion.

Alternative Name

Spike glycoprotein; S glycoprotein; E2;   Peplomer protein

UniProt

Q91AV1

Immunogen

A scFv antibody phage display library was constructed   from peripheral blood lymphocytes of piglets induced with PEDV. The library was screened with four rounds of biopanning using purified PEDV antigen, and   scFv antibodies that bound to PEDV were obtained.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. Immunofluorescence assay   revealed that the scFv stained PEDV-infected Vero E6 cells but did not react   with uninfected control cells. The scFv was shown to inhibit PEDV infectivity   by the plaque reduction neutralization assay (virus neutralization titer =   6.25 µg/mL). Results showed the a cocktail of the scF fragment, PZZ 21 and   PZZ 35 neutralized viral infection at 3.125 µg/mL. Finally, piglets orally   administered with a mixture of scFv fragment, PZZ 21 and PZZ 35 showed no to   mild clinical symptoms, significantly less viral shedding, no mortality and   no intestinal lesions.

Antibody First   Published

Zhang et al. Single Chain Fragment Variable   (scFv) Antibodies Targeting the Spike Protein of Porcine Epidemic Diarrhea Virus Provide Protection against Viral Infection in Piglets. Viruses.2019   Jan; 11(1): 58. PMID:30646521

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.