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Anti-SpuE mAbs

简要描述:

The antibody binds specifically to SpuE. In particular, it interacts with the hinge region that connects the two domains of SpuE.

产品参数:

Cat. No.:MABL-2971

Species:Engineer

Species Reactivity:P. aeruginosa

Type:Recombinate

Application:crystallization, ELISA

详细介绍

Key features and   details


Cat. No.

MABL-2971

Name

Anti-SpuE mAbs

Clone No.

AFD- ScFv5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallization, ELISA

Species Reactivity

P. aeruginosa

Basic Information


Specificity

The antibody binds specifically to SpuE. In   particular, it interacts with the hinge region that connects the two domains   of SpuE.

Alternative Name

Spermidine-binding periplasmic protein SpuE

UniProt

Q9I6J0

Immunogen

The original antibody was isolated from a   human scFv phage-displayed library by panning against SpuE.

Application Notes

The specificity of the original format of   the antibody was confirmed by ELISA analysis. The antibody conjugated to a   membrane penetrating peptide (MPP) could inhibit the secretion of ExoS from   P. aeruginosa. The antibody conjugated to MPP was able to inhibit the   cytotoxic effects of SpuE in the human epithelial cell line A549. The   antibody conjugated to MPP showed a protective effect on C. elegans from P.   aeruginosa infection. The crystal structure of the antibody in complex with   SpuE-spermidine was determined. The dissociation constant (KD) of SpuE to   spermidine and of SpuE to spermidine in the presence of the antibody was   measured by isothermal titration calorimetry (Kd = 2.7 nM and 29.8 nM,   respectively) (Zhang et al., 2019; PMID: 31682834).

Antibody First   Published

Zhang et al., A Potent Anti-SpuE Antibody   Allosterically Inhibits Type III Secretion System and Attenuates Virulence of   Pseudomonas Aeruginosa. J Mol Biol. 2019 Dec 6;431(24):4882-4896. doi:   10.1016/j.jmb.2019.10.026. Epub 2019 Nov 2. PMID:31682834

Note on publication

The original paper describes the generation   and characterization of the antibody.

COA Information For reference only, actual COA shall prevail

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.


 


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