Anti-Sporozoite of Eimeria acervulina mAbs

Cat. No.

MABL-2970

Name

Anti-Sporozoite of Eimeria acervulina mAbs

Clone No.

AFD- 6D-12-G10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IF

Species Reactivity

Eimeria acervulina

Basic Information

Specificity

This antibody recognizes and binds the   apical complex of the sporozoite of Eimeria acervulina, which is a 20-21kDa   protein.

Alternative Name

Sporozoite surface antigen; Eimeria   acervulina

UniProt

Q24769

Immunogen

The original antibody was generated by immunizing   chicken with sporozoite antigen of Eimeria acervulina, which is a 20-21 kDa   protein.

Application Notes

Avain coccidiosis is caused by an   intracellulae protozoan belonging to the genus Eimeria. This disease is   important in aviculture due to its negative effects on production and   development of birds. Avian coccidiosis is one of the main causes of growth   retardation, poor feed conversion and high mortality in intensive farming of   broilers, cage-free pullets and breeders. This mAb recognizes the apical   complex of the sporozoite in a western blot assay and ELISA. Immunoelectron   microscopic examination revealed that the antibody stained the conoid   antigen. In indirect immunofluorescence assay the culture supernatent   containing the mAb intensely stained the tip of the E. acervulina   sporozoites. It is also reported that this antibody inhibited the invasion of   sporozoites in CD8+ T cells in vitro (PMID: 8627507). The scFv antibody was   produced at a minimum of 7 mg l−1 and exhibited virtually identical antigen   reactivity as the original mAb (Min et al., 2001).

Antibody First   Published

Sasai et al.   Characterization of a chicken monoclonal antibody that recognizes the apical   complex of eimeria acervulina sporozoites and partially inhibits sporozoite   invasion of cd8+ t lymphocytes in vitro. J Parasitol (1996); 82(1):82-7.   PMID:8627507

Note on publication

Describes the generation of this antibody   and its use in identifying the Eimeria sporozoite antigen that binds to CD8+   lymphocytes.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.