Key features and details | |
Cat. No. | MABL-3036 |
Name | Anti-Testosterone mAbs |
Clone No. | AFD- TM-4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males. |
Alternative Name | ChEBI:17347 |
UniProt | |
Immunogen | This antibody was raised by panning against a library that has earlier been selected twice for activity against testosterone-biotin(testosterone3-(5-(6-(5- (biotin)pentanamido)hexanamido)pentylcarbamoyl) propanoate) coupled to avidin-coated magnetic beads. |
Application Notes | The binding characteristics of the original version of this antibody (human scFv) to testosterone and biotinylated testosterone were characterized via competitive ELISA. It exhibited an IC50 value of 2 and 4 nM for testosterone and biotinylated testosterone, respectively (Persson et al., 2008; PMID: 18480091). |
Antibody First Published | Persson et al. In vitro evolution of an antibody fragment population to find high-affinity hapten binders. Protein Eng Des Sel. 2008 Aug;21(8):485-93. PMID:18480091 |
Note on publication | The original publication details the in vitro evolution of an antibody fragment population, focusing on enhancing their hapten binding properties, particularly targeting testosterone as a model antigen, resulting in a considerable increase in affinity and the identification of novel variants with different fine-specificities. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
