Key features and details | |
Cat. No. | MABL-3035 |
Name | Anti-Testosterone mAbs |
Clone No. | AFD- S83 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody is specific for the testosterone molecule. Testosterone is the primary sex hormone and anabolic steroid in males. |
Alternative Name | ChEBI:17347 |
UniProt | |
Immunogen | This antibody is an affinity matured version of the murine parent antibody 3C4F5. This antibody was generated by panning against a antibody phage library created using PCR mutagenesis. |
Application Notes | The binding characterization of the antibody was done using ELISA. To show the effectiveness of this antibody, a competitive immunoassay was performed on the serum of unknown patients using the human version of this antibody. It was compared to a commercial testosterone kit. The results were comparable (WO1999012974). The original Fab fragment of this antibody, having 5 optimised CDRs, showed 32-fold higher relative affinity to testosterone, 60-fold lower cross-reactivity to DHEAS, 2 fold lower cross-reactivity to 5α-DHT and over 9-fold lower cross-reactivity to androstenedione compared to the original monoclonal antibody (Ari Hemminki, 1998; ISBN 951-38-5343-8). |
Antibody First Published | Ari Hemminki Development of recombinant antibodies for diagnostic applications by protein engineering. ISBN 951-38-5343-8 |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
