Key features and details | |
Cat. No. | MABL-2977 |
Name | Anti-ssRNA (sequence specific) mAbs |
Clone No. | AFD- BRG (5E08) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assays, structural analysis |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody binds to an RNA fragment of a minimal 10 nt sequence N3U4G5C6A7N8A9G10G11N12. |
Alternative Name | single stranded RNA; RNA; ribonucleic acid |
UniProt | |
Immunogen | This Fab antibody fragment was raised using YSGR phage display library against a branched RNA fragment corresponding in sequence to the branch region of the pre-mRNA intron derived from the YBL059W gene from S. cerevisiae. |
Application Notes | It was demonstrated that the binding of BRP Fab fragment to the RNA molecule stabilised an RNA hairpin structure which was not steady in the absence of the Fab (Shao et al., 2016). Consequently, BRP might be used to capture dynamic RNAs in distinct conformational states and allow structural analysis of nucleic acids. Additionally, BRG Fab antibody fragment was shown to inhibit Dbr1 debranching of the RNA target (Shao et al., 2016). |
Antibody First Published | Shao et al. Specific Recognition of a Single-Stranded RNA Sequence by a Synthetic Antibody Fragment. J Mol Biol. 2016 Oct 9;428(20):4100-4114. doi: 10.1016/j.jmb.2016.08.029. Epub 2016 Sep 2 PMID:27593161 |
Note on publication | This article describes the generation and characterisation of the BRG antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
