Key features and details | |
Cat. No. | MABL-2978 |
Name | Anti-Staphylococcal enterotoxin B mAbs |
Clone No. | AFD- LSEBg6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Staphylococcus aureus |
Basic Information | |
Specificity | This antibody binds enterotoxin B of Staphylococcus aureus (SEB). |
Alternative Name | entB; SEB; Enterotoxin type B |
UniProt | P01552 |
Immunogen | The original antibody was isolated from a non-immune semisynthetic llama library constructed from blood samples (6 ml) collected from the left jugular vein of three research naïve mature llamas. |
Application Notes | The binding specificity of this antibody towards staphylococcal enterotoxin B was checked using ELISA. This antibody can also be used as a detection antibody for SEB in a sandwich ELISA. The original VHH antibody can detect SEB concentrations down to 0.05 ng/ml and 0.5 ng/ml in a sample (PMID:17165813). |
Antibody First Published | Goldman et al. Facile Generation of Heat Stable Antiviral and Antitoxin Single Domain Antibodies from a Semi-synthetic Llama Library. Anal Chem. 2006 Dec 15; 78(24):8245–8255. PMID:17165813 |
Note on publication | A semisynthetic llama library was constructed from non-immune llamas and binders specific for live vaccinia virus (a smallpox virus surrogate), hen egg lysozyme, cholera toxin, ricin, and staphylococcal enterotoxin B were selected. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
