Key features and details | |
Cat. No. | MABL-2984 |
Name | Anti-STAT3 phosphorylated mAbs |
Clone No. | AFD- AbAb1-pSTAT3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | This antibody binds human phosphorylated STAT3. This protein mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. |
Alternative Name | APRF; Signal transducer and activator of transcription 3; Acute-phase response factor |
UniProt | P40763 |
Immunogen | The original antibody was generated by immunizing rabbits with recombinant phosphorylated peptide pSTAT3 (PGSAAP-pY-LKTKGGGSC) conjugated with KLH as an immunogen. |
Application Notes | The binding reactivity of this antibody to recombinant phosphorylated pSTAT3 peptide was confirmed using ELISA. The binding reactivity was further confirmed using western blot, of STAT3 protein IFN-α-treated cells. The scFv version of this antibody was used fused to human IgG1 using a suitable linker for the generation of scFv-Fc mini-antibody. This minibody was capable of immunoprecipitating pSTAT3 in the cell lysate of HeLa cells treated with IFN-α (US20090233358). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
