Key features and details | |
Cat. No. | MABL-3005 |
Name | Anti-Talin 1 mAbs |
Clone No. | AFD- 8D4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, IP, WB, ELISA, FC, IF |
Species Reactivity | Chicken, Rat, Xenopus, Human, Mouse |
Basic Information | |
Specificity | The antibody is specific for mammalian and avian talin. Talin is a multifunctional constituent of cell-substratum attachment sites and it has a high molecular weight (225-235 kDa). The protein is found in a variety of tissues and cell types. |
Alternative Name | talin 1; Talin1; talin-1; TLN; TLN1 |
UniProt | Q9Y490 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with purified cytoskeletal Talin, from chicken gizzard. |
Application Notes | The antibody detected the N-terminal region of talin1 by western blot analysis (Praekelt et al., 2012; PMID: 22306379 and Martel et al., 2001; PMID: 11279249). However, it also detected the equivalent region of talin2, albeit to a lesser degree (Praekelt et al., 2012; PMID: 22306379). This antibody was used for detection of talin expression on HeLa/AT22, AT21, wild-type HeLa cells or pMEP4- transfected HeLa cells cells by flow cytometry (Albigès-Rizo et al., 1995; PMID: 7593292). lmmunocytochemistry using the antibody showed patterns of talin expression within DRG growth cones (Sydor et al., 1996; PMID: 8794861). Indirect immunofluorescence staining of cultured fibroblasts (rat, human, chicken and xenopus embryo fibroblasts) was tested with the antibody. In all the samples the focal adhesions were stained with the antibody (Otey et al. 1990; PMID: 1690175). |
Antibody First Published | Otey et al. Characterization of monoclonal antibodies to chicken gizzard talin Hybridoma. 1990 Feb;9(1):57-62. PMID:1690175 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
