Anti-TCR mAbs

Cat. No.

MABL-3016

Name

Anti-TCR mAbs

Clone No.

AFD- Desire-1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallisation, IP, FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

mAb desire-1 recognises an epitope   contributed by both the alpha and beta chains of the KB5- C20 TCR.

Alternative Name

T cell receptor; T-cell receptor; Ti

UniProt

P01852; P01849

Immunogen

mAb desire-1 was obtained after fusion of   spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in   RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1   was selected for its capacity to inhibit selectively the H-2Kb-specific   cytolysis by clone KB5-C20.

Application Notes

Desire-1 has been used for the   immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986).   An FITC conjugated desire-1 has been used in flow cytometric analysis as a   marker for clonotype expression (Schonrich 1991). Further, a Fab fragment   derived from desire-1 has been used in the crystallisation of the T-cell   receptor for 3D structure determination (Housset 1997).

Antibody First   Published

Hua et al. Lysis of hybridoma cells bearing   anti-clonotypic surface immunoglobulin by clonotype-expressing alloreactive  cytotoxic T cells Eur. J. Immunol. 1985. 15: 1029-1032 PMID:3902483

Note on publication

Describes the ability desire-1 to modulate the   cytolytic activity of cytotoxic T lymphocyte clone KB5-C20.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.