Key features and details | |
Cat. No. | MABL-3017 |
Name | Anti-TCR mAbs |
Clone No. | AFD- H57-597 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallisation, IP, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | mAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain. |
Alternative Name | TCR-beta; T cell receptor; T-cell receptor; T cell receptor beta chain; TCRB; TRB; TCB1; Ti |
UniProt | P01852 |
Immunogen | Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti- TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas. |
Application Notes | In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006). |
Antibody First Published | Kubo et al. Characterisation of a monoclonal antibody which detects all murine alpha-beta T cell receptors J Immunol. 1989 Apr 15;142(8):2736-42. PMID:2467936 |
Note on publication | Describes the need for a monoclonal antibody that detects alpha-beta but not delta- gamma TCRs, to allow enumeration of the two types of cells separately during development. The generation of the antibody is then outlined. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
