Anti-TCR beta mAbs

Cat. No.

MABL-3018

Name

Anti-TCR beta mAbs

Clone No.

AFD- IARC307

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, FC, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for Vbeta8.1 CDR1   sequence CKPISGHNSLFQWYRQT.

Alternative Name

IARC-307; TCR-beta; T cell receptor; T-cell   receptor; T cell receptor beta chain; TCRB; TRB; TCB1; Ti; TCR V beta;   IARC/307; IARC 307

UniProt

P01850; A0A5B9

Immunogen

Derived from normal B cells in peripheral   blood of a Burkitt’s lymphoma patient. Screened more than 60 culture   supernatants from lymphoblastoid cell lines of normal origin, Epstein-Barr   virus (EBV)- carrying Burkitt lymphomas, EBV-negative Burkitt lymphomas and   EBV-converted sublines of original EBV- negative Burkitt lymphoma human   B-cell lines for their capacity to bind the synthetic peptide autoantigens.   One lymphoblastoid B-cell line was identified, IARC307, that produces IgM   kappa antibodies which specifically react with the CDR1 peptide corresponding   to human Vbeta8.1 gene sequence.

Application Notes

IARC307 has been used in ELISAs to bind to synthetic peptides of CRD1 region from several TCR Vbeta families of man and   mouse (Marchalonis 2000).

Antibody First   Published

Dedeoglu et al. Light and heavy chains   specifying a human IgM kappa autoantibody to a T-cell receptor Vbeta-antigen   Immunology Letters, 38 (1993)223-227 PMID:8125530

Note on publication

Describes the generation of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.