Key features and details | |
Cat. No. | MABL-3026 |
Name | Anti-Tenuazonic acid mAbs |
Clone No. | AFD- 3F9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | bioluminescent enzyme immunoassay, chemiluminescent enzyme immunoassay, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | This antibody is specific for tenuazonic acid, an Alternaria mycotoxin. The antibody does not cross-react with AOH, AME, DON, ITeA, or ZEN. |
Alternative Name | Nb-3F9; TeA; Alternaria mycotoxin tenuazonic acid; (5S)-3-Acetyl-5-[(2S)- butan-2-yl]-4-hydroxy-1,5-dihydro-2H-pyrrol-2-one |
UniProt | |
Immunogen | The original antibody was generated by immunizing a male Bactrian camel with TeA-CMO- KLH followed by antibody library construction and phage display-based selection. |
Application Notes | The activity and sensitivity of the original format of this antibody (VHH) were assessed by ic-ELISA. Nb-3F9-Nluc, a biofunctional nanobody fusion, was generated and its nanoluciferase catalytic activity, nanobody binding activity, and specific TeA inhibition activity were analyzed. The performance of Nb-3F9-Nluc and the original Nb-3F9 was evaluated using two-step ELISA with HRP-anti-6×His tag secondary antibody. The IC50 value of ELISA based on Nb-3F9-Nluc was 80.3 ng/mL, and that for Nb-3F9 was 89.5 ng/mL. Additionally, Nb-3F9 and Nb-3F9-Nluc were used, respectively, in the development and optimization of chemiluminescent enzyme immunoassay and bioluminescent enzyme immunoassay (Wang et al., 2020; PMID: 32702970). TeA is a tetrameric acid derivative, and it is considered to have the highest toxicity and exposure among the Alternaria toxins. The contamination of TeA in foods has widely been a concern for food safety control. |
Antibody First Published | Wang et al. Chemiluminescent Enzyme Immunoassay and Bioluminescent Enzyme Immunoassay for Tenuazonic Acid Mycotoxin by Exploitation of Nanobody and Nanobody- Nanoluciferase Fusion Anal Chem. 2020 Sep 1;92(17):11935-11942. doi:32702970 10.1021/acs.analchem.0c02338 PMID: |
Note on publication | The original publication describes the development of highly specific nanobodies (Nbs), particularly Nb-3F9, against the Alternaria mycotoxin tenuazonic acid (TeA) using a stringent biopanning strategy. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
