Key features and details | |
Cat. No. | MABL-3028 |
Name | Anti-Testosterone mAbs |
Clone No. | AFD- 3C4F5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The antibody binds testosterone and shows reasonably low cross-reaction (~11.6%) with 5a- dihydrotestosterone, androstenedione and ~1% cross reaction with dehydroepiandrosteronesulphate (DHEAS). Testosterone is the primary sex hormone and anabolic steroid in males. |
Alternative Name | ChEBI:17347 |
UniProt | |
Immunogen | The original mouse version of this antibody was raised by immunizing mice with a testosterone-3-carboxymethyloxime-thyroglobulin conjugate. |
Application Notes | The original mouse IgG2b version of this antibody binds testosterone with an affinity of 0.3 nM. The binding properties of the antibody was preliminarily characterized by a competitive enzyme- linked immunosorbent assay. The combined (44 + 28) mutant derived from this antibody showed less cross- reactivity towards DHEAS of 0.033%, 44-fold less than in the wild-type (Hemminki et al, 1998; pmid:9680193). The combined mutant is available on request. To elucidate the structure of the mouse version of this antibody bound to testosterone x-ray crystallography was performed (Valjakka et al, 2002; pmid:11707437). |
Antibody First Published | Hemminki et al. Specificity improvement of a recombinant anti-testosterone Fab fragment by CDRIII mutagenesis and phage display selection. Protein Eng. 1998 Apr;11(4):311-9. PMID:9680193 |
Note on publication | The specificity of a recombinant anti-testosterone Fab fragment was improved by CDRIII mutagenesis and phage display selection. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
