Anti-TIGIT mAbs

Cat. No.

MABL-3072

Name

Anti-TIGIT mAbs

Clone No.

AFD- 1B4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, ELISA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine TIGIT.

Alternative Name

VSTM3; T cell Ig and ITIM domain; T cell immunoreceptor with Ig and ITIM domains; V-set and transmembrane   domain-containing protein 3; WUCAM

UniProt

P86176

Immunogen

This antibody was raised by immunising TIGIT-/-   mice with recombinant murine TIGIT tetramers.

Application Notes

The binding specificity of this antibody for   TIGIT has been confirmed in ELISA analysis and by flow cytometry using P815   cells transfected with mouse TIGIT and activated mouse splenocytes (Dixon et   al, 2018). This antibody also specifically stains activated primary wild-type   T cells (Dixon et al, 2018). This antibody fully blocks CD155 binding to   TIGIT, and acts as a TIGIT blocking antibody in vivo. Administration of this   antibody increases experimental autoimmune encephalomyelitis (EAE) severity,   and retards the growth of established MC38 colon carcinoma and GL261   glioblastoma (Dixon et al, 2018). This antibody synergises with anti-PD-1   blocking antibodies to achieve complete tumour regression in colon carcinoma   models (Dixon et al, 2018). The original mouse IgG1 antibody does not deplete   TIGIT-expressing CD4+ or CD8+ T cells in vivo, nor affect T cell   proliferation in vitro (Dixon et al, 2018).

Antibody First   Published

Dixon et al. Functional Anti-TIGIT   Antibodies Regulate Development of Autoimmunity and Antitumor Immunity J   Immunol. 2018 Apr 15;200(8):3000-3007 PMID:29500245

Note on publication

Describes the original generation of this   antibody, and the establishment of its TIGIT- blocking function.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.