Key features and details | |
Cat. No. | MABL-3096 |
Name | Anti-Tissue factor mAbs |
Clone No. | AFD- hTFFab148H |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human tissue factor (TF) a hemostatic protein which is involved in the extrinsic pathway of coagulation. TF is receptor for circulating serine protease factor VIIa, activating the catalytic VIIa protease domain. The complex formed functions to activate the zymogen factor X into activated factor Xa, which then catalyzes the formation of Prothrombin to Thrombin. The pathways results in a fibrin clot which acts to stop bleeding. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. |
Alternative Name | CD142; TF; hTF; F3; Coagulation factor III; Thromboplastin; platelet tissue factor; factor III |
UniProt | P13726 |
Immunogen | The original antibody was isolated from a human Fab antibody library generated from peripheral blood collected from 300 healthy individuals. |
Application Notes | The binding characterization of this Fab antibody was done using ELISA and dot blot. This antibody shows positive plasma coagulation inhibitory activity. This antibody is capable of neutralizing human tissue factor in vitro by inhibiting the binding of TF factor/Vlla complex to factor X. The inhibition rate increase with the concentration. The pharmacodynamic and pharmacokinetic activity of this antibody was studied in vivo in rat models (CN101423552). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
