Key features and details | |
Cat. No. | MABL-3097 |
Name | Anti-TL1A mAbs |
Clone No. | AFD- chTL1A-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, neutralizing, WB, ELISA |
Species Reactivity | Chicken |
Basic Information | |
Specificity | The antibody binds specifically to chicken TL1A, a type II transmembrane protein predominantly expressed by endothelial cells that promotes the expansion of activated T cells and regulatory T cells, modulates inflammation, and regulates the production of a wide variety of T cell cytokines. The antibody does not react with the chIL-8/pET32a or proteinase K-pretreated chTL1A, verifying its specificity for the intact chTL1A. |
Alternative Name | TNFSF15; TNF-like ligand 1A; tumor necrosis factor superfamily 15; chicken TL1A; Tumor necrosis factor-like ligand 1A; chTL1A; TL1A mAb-1 |
UniProt | Q50L61 |
Immunogen | The original antibody was raised by immunizing BALB/c mice with recombinant TL1A protein. |
Application Notes | The specificity of the original antibody was confirmed by ELISA analysis (Lee et al, 2014; pmid: 24565040). The antibody was used as a capture antibody in capture ELISA assay to measure serum TL1A levels in normal and necrotic enteritis (NE)-afflicted chickens. The antibody detected the recombinant chicken TL1A by western blot analysis. The antibody identified TL1A-secreting cells in the chicken thymus, cecal tonsil, and bursa of Fabricius by immunocytochemistry. Furthermore, the antibody inhibited chTL1A induced spleen lymphocyte proliferation, nitric oxide production by chicken macrophage cells (HD11), and blocked the cytotoxic effect of chTL1A against lymphoblastoid chicken B tumor cells (LSCC-RP9) (Lee et al, 2014; pmid: 24565040). |
Antibody First Published | Lee et al. Development and characterization of mouse monoclonal antibodies reactive with chicken TL1A. Vet Immunol Immunopathol. 2014;159(1-2):103-9. PMID:24565040 |
Note on publication | The paper describes the generation and characterization of the antibody, specific for chicken TL1A. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
