Anti-VEGFR2 mAbs

Cat. No.

MABL-3201

Name

Anti-VEGFR2 mAbs

Clone No.

AFD- B1C4A7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, in vivo, SPR, ELISA

Species Reactivity

Human

Basic Information

Specificity

The antibody is specific for VEGFR2.

Alternative Name

flk-1;FLK-1; KDR; Fetal liver kinase 1;   Kinase ｉｎｓｅｒｔ domain receptor; Protein-tyrosine kinase receptor; EC:2.7.10.1;   Vascular endothelial growth factor receptor 2; Vascular endothelial growth   factor receptor

UniProt

P35968

Immunogen

The original antibody was isolated by   panning a phage display library against VEGFR2.

Application Notes

The antibody, together with dsD7A8, was used   for constructing the bispecific antibody (A7-A8)-LALA. The original and the bispecific antibody could strongly bind to the recombinant human VEGFR2 and   mouse VEGFR2 (EC50 =0.107 nM and 0.466 nM for B1C4A7 and 0.128 nM and 0.598   nM for A7- A8 respectively). The bispecific antibody could strongly block   ligand VEGF binding to its receptor VEGFR2 (IC50 for hVEGF-hVEGFR2   interaction was 1.29 nM for the bispecific antibody and 1.72 nM for B1C4A7   and IC50 for mVEGF-mVEGFR2 interaction was 0.801 nM for the bispecific   antibody and 0.973 nM for B1C4A7). The original and bispecific antibodies   could bind to KDR/PAE cell expressed human VEGFR2, mouse VEGFR2 (EC50 =0.522   nM and 0.533 nM, respectively). The bispecific antibody associated to VEGFR2   fast and dissociated slowly (KD = 47 pM for the bispecific and 752 pM for the   original antibody).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.