Anti-VIM-4 MβL mAbs

Cat. No.

MABL-3207

Name

Anti-VIM-4 MβL mAbs

Clone No.

AFD- NbVIM_38

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibition, ELISA

Species Reactivity

Basic Information

Specificity

This antibody is specific for VIM-4 M beta   L. The main epitope of this antibody is amino acid 99 to 133.

Alternative Name

UniProt

Immunogen

The original antibody was raised by panning a   dromedary phage display library against VIM-4 M beta L.

Application Notes

The epitope of this antibody was determined using ELISA on different parts of its antigen. The ability of this antibody   to inhibit VIM-4 was shown. The presence of this antibody inhibited activity   of VIM-4 against imipenem, benzylpenicillin and cephalothin (Sohier et al.,   2013; PMID:23289540).

Antibody First   Published

Sohier et al. Allosteric inhibition of VIM   metallo-β-lactamases by a camelid nanobody Biochem J. 2013 Mar   15;450(3):477-86. PMID:23289540

Note on publication

The original paper used an approach on the   basis of phage display was used to select single-domain antibody fragments   (VHHs, also called nanobodies) that would inhibit the clinically relevant VIM   (Verona integron-encoded MβL)-4 MβL.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.