Key features and details | |
Cat. No. | MABL-3208 |
Name | Anti-Vimentin mAbs |
Clone No. | AFD-1433 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IF |
Species Reactivity | Human, dog, rat |
Basic Information | |
Specificity | This antibody binds the vimentin intermediate filament. Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. |
Alternative Name | VIM |
UniProt | P08670 |
Immunogen | The original antibody was generated from an immunized alpaca. |
Application Notes | This chimeric engineered antibody is generated by fusing the original alpaca derived nanobody to mouse IgG1 and rabbit Fc domains to extend the research application of the original. This antibody binds vimentin present on canine MDCK cells, rodent BHK cells and on human A549 cells upon stimulation with TGFβ. This antibody can be used for immunofluorescent staining of tissues expressing vimentin. It can also be conjugated with fluorophores for direct detection. It can also be immobilized on protein A, beads, resins and plates. The original alpaca VHH antibody is available from ChromoTek. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
