Key features and details | |
Cat. No. | MABL-3211 |
Name | Anti-Vimentin mAbs |
Clone No. | AFD- RV202 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IHC |
Species Reactivity | canine, Caprine, Chicken, Hamster, Monkey, Rat, Swine, Xenopus, Zebrafish, Human, Mouse |
Basic Information | |
Specificity | The antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. |
Alternative Name | |
UniProt | P08670 |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with a cytoskeletal vimentin extract of calf lens. |
Application Notes | The antibody detected vimentin by western blot analysis. This antibody was used for vimentin detection on rabbit embryo, dermatome, myotome (adjacent abdominal wall) and the genital ridge (comprising mesonephric blastema and mesonephric duct) by immunohistochemistry (Viebahn et al., 1988; PMID: 2460241). The original antibody detected vimentin on HeLa cells transfected with pRSVDes by immunohistochemistry (Raats et al., 1990; PMID: 1699950). |
Antibody First Published | Ramaekers et al. Tissue distribution of keratin 7 as monitored by a monoclonal antibody Exp Cell Res. 1987 May;170(1):235-49. PMID:2436934 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
