Key features and details | |
Cat. No. | MABL-3212 |
Name | Anti-Vimentin mAbs |
Clone No. | AFD- RV203 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, FC, IHC |
Species Reactivity | canine, Caprine, Hamster, Rat, Swine, Human |
Basic Information | |
Specificity | The antibody reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. |
Alternative Name | |
UniProt | P08670 |
Immunogen | The original antibody was generated by immunizing a BALB/c mouse with a cytoskeletal vimentin extract of calf lens. |
Application Notes | The antibody detected vimentin on cytoskeletal extracts of bovine lens, BHK-21 and HeLa cells by western blot analysis. The antibody detected vimentin on connective tissue fibroblasts of hamster tongue, polygonal BHK-21/C13 cells and elongated BHK-21 cells by immunohistochemistry (Schaart et al., 1991; PMID: 2065865). |
Antibody First Published | Schaart et al. Baby hamster kidney (BHK-21/C13) cells can express striated muscle type proteins Differentiation. 1991 Mar;46(2):105-15. PMID:2065865 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
