Anti-VSIG4 mAbs

Cat. No.

MABL-3223

Name

Anti-VSIG4 mAbs

Clone No.

AFD- Nb119

From

Recombinant Antibody

Isotype

Engineer antibody

Application

crystallisation, SPR, WB, FC

Species Reactivity

Human, Mouse

Basic Information

Specificity

Nb119 is able to cross react with both murine   and human VSIG4, showing a 250-fold higher affinity with the mouse protein   (Wen et al, 2017). Shown crystallographically, Nb119 binds to the variable   immunoglobulin domain of both murine and human VSIG4 at the same epitope,   contacting beta-strands at the IgV C-terminus. VSIG4 is a dual-functional   regulator of T cell immune response and complement receptor. It is a   molecular marker of liver disease and arthritis in mice. Recently, it has   been found overexpressed on tumour-associated macrophages, and may play a   role in non-small cell lung cancer tumourigenesis.

Alternative Name

Z39Ig; V-set and immunoglobulin   domain-containing protein 4; Complement receptor of the Ig superfamily; CRIg;   Protein Z39Ig; Z39Ig; Vsig4

UniProt

Q9Y279

Immunogen

The extracellular domain of   recombinantly-produced mouse VSIG4 was used to immunise an alpaca (Vicugna   pacos). Peripheral blood lymphocytes isolated from the immunised alpaca were   used to generate a Nanobody phage library, clones from which were selected   for binding to the same recombinant mouse VSIG4 extracellular domain.

Application Notes

Nb119 was shown to exhibit an affinity for murine VSIG4 of 0.9 nM by SPR (Zheng et al, 2014) or 3.5 nM by ITC (Wen et al, 2017). It was further shown by flow cytometry that Nb119 recognised mouse VSIG4 expressed on liver macrophages. Nb119 was crystallised in complex with   both murine and human VSIG4 (Wen et al, 2017).

Antibody First   Published

Zheng et al. Molecular Imaging with   Macrophage CRIg-Targeting Nanobodies for Early and Preclinical Diagnosis in a   Mouse Model of Rheumatoid Arthritis J Nucl Med. 2014 May;55(5):824-9.   PMID:24686780

Note on publication

Describes the generation of the Nb119   Nanobody from a phage library against murine VSIG4. The Nanobody was   subsequently radiolabelled and used for live imaging in arthritic mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.