Key features and details | |
Cat. No. | MABL-3222 |
Name | Anti-VP1694 mAbs |
Clone No. | AFD- FA7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Vibrio parahaemolyticus |
Basic Information | |
Specificity | This antibody is specific for the VP1694 protein from Vibrio parahaemolyticus. This proteins is necessary for this bacteria to act as a pathogen. Vibrio parahaemolyticus can cause gastroenteritis in humans. |
Alternative Name | Type III export protein YscF |
UniProt | Q87P24 |
Immunogen | This antibody was generated by panning a scFv library from BALB/c mice immunized with purified fusion protein against GST-VP1694 antigen. |
Application Notes | An ELISA was performed on associated V. parahaemolyticus proteins (TDH, TLH, VopQ, VP1694), Homologous protein HrpA, and PBS using the scFv version of this antibody. It showed a stronger binding to VP1694 than to other proteins. Furthermore, a western blot was performed on VP1694 using this antibody. The western blot showed the binding of VP1694 by the antibody (Wang et al, 2014; pmid:25320414). |
Antibody First Published | Wang et al. Generation and Characterization of a scFv Antibody Against T3SS Needle of Vibrio parahaemolyticus. Indian J Microbiol. 2014 Jun; 54(2): 143–150. PMID:25320414 |
Note on publication | A phage single-chain fragment variable antibody (scFv) library was constructed, and a specific scFv against VP1694 named scFv-FA7 was screened by phage display panning. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
