Key features and details | |
Cat. No. | MABL-3221 |
Name | Anti-von Willebrand factor mAbs |
Clone No. | AFD- 82D6A3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vivo, inhibition |
Species Reactivity | Baboon, Human |
Basic Information | |
Specificity | This antibody is specific for human vWF, A3 domain. |
Alternative Name | human vWF A3 domain; VWF-A3; vWF |
UniProt | P04275 |
Immunogen | The original antibody was purified from murine ascites by Protein A chromatography. |
Application Notes | This antibody was used as an inhibitor to study platelet adhesion to collagen VI and ECM. It was found to inhibit the binding of von Willebrand factor (VWF) to collagen I and III (Hoylaerts et al., 1997; PMID: 9164855). This antibody was found to have a comparable affinity for baboon vWF as for human vWF and inhibited the binding of baboon vWF to collagen with an inhibitory concentration of 50% (IC50) of 3.5 μg/ml. Furthermore, it prevented the binding of VWF to collagen and removed bound VWF from a collagen surface, albeit with a lower efficacy. Moreover, the antithrombotic efficacy of the antibody was evaluated in a modified Folts model in baboons; this antibody was used to inhibit the interaction between collagen and vWF to prevent arterial thrombosis in baboons (Wu et al., 2002; PMID: 11986216). The crystal structure of the antibody's Fab fragment, in complex with the vWF A3 domain, was determined (Staelens et al., 2006; PMID: 16314412). |
Antibody First Published | Hoylaerts et al. von Willebrand factor binds to native collagen VI primarily via its A1 domain Biochem J. 1997 May 15;324 ( Pt 1)(Pt 1):185-91. doi: 10.1042/bj3240185 PMID:9164855 |
Note on publication | The original publication investigates the interaction mechanism between collagen VI and von Willebrand factor (vWF). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
