Anti-Vitellogenin mAbs

Cat. No.

MABL-3220

Name

Anti-Vitellogenin mAbs

Clone No.

AFD- H4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Chinese rare minnow (Gobiocypris rarus),   common carp (Cyprinus carpio), crucian carp (Carassius auratus), zebrafish   (Danio rerio)

Basic Information

Specificity

This antibody binds a linear epitope of   vitellogenin (Vtg) at the N-terminal region of the protein from zebrafish,   Chinese rare minnow, common carp and crucian carp. In female oviparous   animals, vitellogenin (Vtg), the phospholipoglycoprotein precursor to the   major egg yolk protein, is synthesized in the liver in response to endogenous estrogen. Vtg synthesis in males can be used as a biomarker for exposure to   estrogen and estrogen mimics in males where Vtg levels are normally low but can be increased upon exposure.

Alternative Name

Vtg; Vtg1; VG; Vitellogenin 1

UniProt

Q90YN8

Immunogen

The original antibody was isolated from an   scFv phage display library generated from the splenic mRNA from 100 non-mmunized   6 wk old female BAL b/c mice after three rounds of panning.

Application Notes

The scFv version of this antibody can bind   vitellogenin (Vtg) from various cyprinid fish species like zebrafish, Chinese   rare minnow and common carp in an ELISA. This antibody can also recognize   denatured 28a-VI polypeptide in western blotting. This antibody was used in   the analysis of Vtg in plasma samples from zebrafish exposed to a range of   concentrations of 17α- ethynyl estradiol (EE2). This antibody can be used in   ELISA to measure Vtg in serum samples from common carp, zebrafish and rare   minnow with similar binding specificities and sensitivities. The sensitivity   of detection for this antibody was found to be 50 ng Vtg/ml (PMID: 21290899).

Antibody First   Published

Rao et al. Novel recombinant monoclonal   antibodies for vitellogenin assays in cyprinid fish species. Dis Aquat Organ.   2010 Dec 7;93(1):83-91. PMID:21290899

Note on publication

Describes the production of H4 scFv a from   phage display library and the characterization of the scFv by ELISA and WB to determine binding specificity and binding curves.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.