Anti-Vitellogenin mAbs

Cat. No.

MABL-3219

Name

Anti-Vitellogenin mAbs

Clone No.

AFD- B5

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA

Species Reactivity

Chinese rare minnow, common carp, crucian carp, Zebrafish

Basic Information

Specificity

B5 recognises a linear epitope of Vtg at the   N-terminal region of the protein from zebrafish, Chinese rare minnow, common carp and crucian carp. Vtg is the phospholipoglycoprotein precursor to the major egg yolk protein which is synthesized in the liver in response to endogenous estrogen in females. Vtg synthesis in males can be used as a biomarker for exposure to estrogen and estrogen mimics in males where Vtg   levels are normally low but can be increased upon exposure.

Alternative Name

Vtg

UniProt

Q90YN8

Immunogen

B5 was isolated from a phage display library constructed using spleenic mRNA from non- immunized mice after 3 rounds of panning.

Application Notes

B5 scFv can be used in WB and ELISA studies.

Antibody First   Published

Rao et al. Novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species Dis Aquat Organ.   2010 Dec 7;93(1):83-91. PMID:21290899

Note on publication

Describes the production of B5 scFv a from   phage display library and the characterisation of the scFv by ELISA and WB to determine binding specificity and binding curves. A 152aa N-terminal protein fragment from Vtg from different cyprinid fish species was used in this   study.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.