Anti-VISTA mAbs

Cat. No.

MABL-3218

Name

Anti-VISTA mAbs

Clone No.

AFD- 13F3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, ELISA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine VISTA.

Alternative Name

PD-1H; V-domain Ig suppressor of T cell   activation; V-type immunoglobulin domain-containing suppressor of T-cell   activation; Platelet receptor Gi24; V-set domain- containing immunoregulatory   receptor; V-set immunoregulatory receptor; Vsir; Dies1

UniProt

Q9D659

Immunogen

This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig   fusion protein.

Application Notes

The specificity of this antibody has been   confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017; Sergent et al, 2018) and mice   infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to   block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011).   In mice models, this antibody exacerbates experimental autoimmune   encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease   progression (Sergent et al, 2018). The use of this antibody to achieve a   VISTA blockade enhances anti-tumour immune responses in mice models of both   transplantable and inducible melanoma, suppressing tumour growth (LeMercier   et al, 2014).

Antibody First   Published

Wang et al. VISTA, a novel mouse Ig superfamily ligand that negatively regulates T cell responses. J Exp Med.   2011 Mar 14;208(3):577-92. PMID:21383057

Note on publication

Describes the original generation of this antibody, and its exacerbation of EAE disease progression.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.