Key features and details | |
Cat. No. | MABL-3226 |
Name | Anti-VSV-G mAbs |
Clone No. | AFD- 1E9F9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, IF |
Species Reactivity | Vesicular stomatitis Indiana virus |
Basic Information | |
Specificity | The IE9F9 (I14) monoclonal antibody reacts with folded VSV-G protein from the Indiana serotype, and has been successfully used in experiments with VSV-G TS045. |
Alternative Name | VSV GP; vesicular stomatitis virus glycoprotein; glycoprotein; Glycoprotein; vcv-glycoprotein; vsv; vesicular stomatitis virus; Ie9F9; I14; G; G protein; G-protein; vesicular stomatitis Indiana virus; vesicular stomatitis Indiana virus Glycoprotein; VSV-Ind G; IE9F9 |
UniProt | |
Immunogen | The antibody was raised by immunizing mice with VSV. |
Application Notes | IF: 1E9F9 anti-VCV-G antibody was used to detect cell-surface levels of VSV G-protein in studies on the rate of cellular transport in amyotrophic lateral sclerosis cellular model (Genevini et al., 2014). IF and WT: Yonemura et al. (2016) successfully used 1E9F9 antibody to perform immunofluorescence staining of HeLa cells which had been prevoiusly tranfected with VSVG-EYFP and detected VCVG protein on Western blots. IF: In folding experiments, 1E9F9 antibody stained VSVG in HEK293 cells transfected with VSVG-GFP (Tam et al., 2018). |
Antibody First Published | Lefrancois and Lyles The interaction of antibody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology. 1982 Aug;121(1):157-67. PMID:18638751 |
Note on publication | The article describes the method of generation of various antibodies against surface glycoprotein of two serotypes of the VSV: Indiana and New Jersey. Among them there is the IE9F9 (I14) antibody. The paper further characterises those antbodies and analyses their epitopes in relation to one another. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
