Key features and details | |
Cat. No. | MABL-3228 |
Name | Anti-VSV-G mAbs |
Clone No. | AFD- M55 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralization, ELISA |
Species Reactivity | Vesicular stomatitis Indiana virus |
Basic Information | |
Specificity | This antibody reacts with a defined subsite within the major antigenic site of VSV-G. The exact epitope is not defined. |
Alternative Name | Glycoprotein G; Glycoprotein; G |
UniProt | P0C2X0 |
Immunogen | The original antibody was generated by immunizing SCID BALB/c mice with vesicular stomatitis virus (VSV). |
Application Notes | This stable, recombinant single-chain Fv-Cκ (scFv-Cκ) antibody fragment was generated by protoplast fusion of J558L myeloma cells with Escherichia coli K803 transformed with an appropriate scFv-Cκ expression construct. The aptly named M55 scFv-Cκ expression construct — containing a CDR2 Ser55-to-Arg substitution (T-to-G transition at the third base), found in the VH chain of several hypermutated antibodies (VI22, VI43, and VI53) — was derived from the secondary antibody clone VI24. M55 binds a defined subsite within the major antigenic site of VSV-G. However, the exact binding site in the subunit is not defined. M55 binding to VSV-G was measured using solid-phase ELISA. The VH Ser55-to-Arg substitution in M55, which is not found in its parental clone VI24, increased monovalent binding by about 10-fold compared with M0 (the germline derived scFv-Cκ). |
Antibody First Published | Kalinke et al. Virus neutralization by germ-line vs. hypermutated antibodies Proc Natl Acad Sci USA. 2000 Aug 29;97(18):10126-31. doi: 10.1073/pnas.97.18.10126 PMID:10963674 |
Note on publication | The original publication describes the creation of scFv-Cκs (including M55), examines their structure, and evaluates their binding and neutralizing capabilities against VSV-G. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
