Anti-XRCC2 mAbs

Cat. No.

MABL-3249

Name

Anti-XRCC2 mAbs

Clone No.

AFD- 7B7/3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific to human XRCC2 (it   does not bind to other RAD51 paralogs). XRCC2 is a paralog of RAD51 which is   a homologue of RecA (E. coli). It is engaged in homologous recombination   repair (HRR) pathway of double-stranded DNA of chromosomal fragmentations and   deletions.

Alternative Name

XRay Repair Cross Complementing 2; XRay   Repair Cross Complementing Protein 2; DNA repair protein XRCC2; X-ray repair complementing defective repair in Chinese hamster cells 2; XRCC2 7B7; 7B7

UniProt

O43543

Immunogen

This antibody was raised by immunising mice with   the denaturated recombinant human XRCC2 protein which had been previously   amplified and purified from E. coli.

Application Notes

WB: XRCC2 7B7/3 (7B7) antibody was used to visualise XRCC2 on Western blots in multiple experiments (e.g. after previous   gel filtration of infected pf9 cells; after immunoprecipitation with   different antibodies and others) in a study on the RAD51 paralogs' complexes   and their role in genetic recombination (Masson et al., 2001).

Antibody First   Published

Masson et al. Identification and   purification of two distinct complexes containing the five RAD51 paralogs.   Genes Dev. 2001 Dec 15;15(24):3296-307. PMID:11751635

Note on publication

This article describes the earliest use of   XRCC2 7B7/3 (7B7/3) antibody to detect XRCC2 protein via Western blotting.   However, the exact process of generation of this antibody is described in the   following paper (where similar antibodies were generated): Masson, J. Y.,   Stasiak, A. Z., Stasiak, A., Benson, F. E., & West, S. C. (2001). Complex   formation by the human RAD51C and XRCC3 recombination repair proteins.   Proceedings of the National Academy of Sciences, 98(15), 8440-8446.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.