Anti-XRCC3 mAbs

Cat. No.

MABL-3250

Name

Anti-XRCC3 mAbs

Clone No.

AFD- 10F1/6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ICC/IF, WB

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for XRCC3 and does   not cross-react with Rad51B, Rad51C, Rad51D, Rad51, or XRCC2 in Western blot   analysis. XRCC3 is a protein involved in the homologous recombination repair   (HRR) pathway in chromosomal fragmentations and deletions.

Alternative Name

X-Ray Repair Cross Complementing 3; X-ray repair cross-complementing protein 3; DNA repair protein XRCC3; X-ray repair complementing defective repair in Chinese hamster cells 3; CMM6; 10F1

UniProt

O43542

Immunogen

This antibody was raised by immunising mice   with the denaturated recombinant human XRCC3-His6 protein which had been   purified from 2.4 liters of E. coli FB810 recA− pLysS carrying pXRCC3- 28c.

Application Notes

WB: XRCC3 10F1/6 antibody was used to   visualise XRCC3 on Western blots in multiple experiments (e.g. after previous gel filtration of infected pf9 cells; after immunoprecipitation with   different antibodies and others) in a study on the RAD51 paralogs' complexes   and their role in genetic recombination (Masson et al., 2001). WB: Sage et   al. (2010) used this antibody to visualise XRCC3 protein on Western blots   from lysates of HCT116 cells in the experiments demonstrating engagement of   Rad51 proteins in mtDNA maintenance. ICC/IF: XRCC3 protein was immunostained   with XRCC3 10F1/6 antibody in HEK293 cells (some of the cells had been   subjected to the ionising radiation) in an experiment investigating the exact   role of XRCC3 in homologous recombination (Forget et al., 2004).

Antibody First   Published

Masson et al. Complex formation by the human   RAD51C and XRCC3 recombination repair proteins. Proc Natl Acad Sci U S A.   2001 Jul 17;98(15):8440-6. PMID:11459987

Note on publication

This article describes the detailed generation of this anti-XRCC3 antibody as well as successfully applies it in   Western analysis.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.