Key features and details | |
Cat. No. | MABL-3271 |
Name | Anti-ZIKV EDII mAbs |
Clone No. | AFD- G9E |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralizing, WB, ELISA |
Species Reactivity | Zika Virus |
Basic Information | |
Specificity | This antibody binds to ZIKV and does not cross-react with DENV4, which was tested as a representative DENV serotype. Its epitope is distinct from the A9E anti-ZIKV antibody. |
Alternative Name | Zika envelope protein DII; ZIKV; Zika virus EDII; EDII |
UniProt | |
Immunogen | This antibody was generated using 6XL method (Kwakkenbos et al., 2010). Memory B cells were isolated from a subject previously infected with ZIKV. After identification of ZIKV-specific monoclonal cultures, genetic material of the variable domains was isolated and recombinant IgG1 lambda antibody forms were grafted with the sequences. |
Application Notes | This antibody has very strong neutralizing properties against Asian and African lineages of ZIKV (Collins et al., 2019). Furthermore, this antibody was effective in preventing Zika infection when administered from one day before the infections in mice (Collins et al., 2019). Therefore, A9E is recommended in development of diagnostic assays which differentiate ZIKV from other flaviviruses (it does not crossreact with DENV) and in the development of therapeutics and vaccines against ZIKV. |
Antibody First Published | Collins et al. Human antibody response to Zika targets type-specific quaternary structure epitopes. JCI Insight. 2019 Apr 18;4(8). Pii: 124588. doi: 10.1172/jci.insight.124588. eCollection 2019 Apr 18 PMID:30996133 |
Note on publication | This article describes the generation and characterization of the G9E antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
