Anti-4-1BBL mAbs

Cat. No.

MABL-317

Name

Anti-4-1BBL mAbs

Clone No.

AFD-AT113-2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, WB, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for 4-1BB   ligand (also known as CD137 ligand), which is a member of the TNF superfamily   and is a type II membrane protein. 4-1BB ligand is a co-stimulatory molecule   that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B   cells) and is upregulated upon activation of these cells. Both of the 4-1BB   ligand and its receptor, TNFRSF9/4-1BB, are involved in the antigen   presentation process and in the generation of cytotoxic T cells. In direct   ELISAs, no cross‑reactivity with recombinant human RELT is observed.

Alternative Name

CD137L; TNFSF9; 4-1BB ligand; 41BBL;   CDw137L; homolog of mouse 4- 1BB-L; receptor 4-1BB ligand; tumor necrosis   factor (ligand) superfamily member 9; tumor necrosis factor (ligand)   superfamily, member 9; tumor necrosis factor ligand 5A; Tumor necrosis factor   ligand superfamily member 9.

UniProt

P41274

Immunogen

This antibody was raised by immunising LOU rats with soluble recombinant 4-1BBL:Fc fusion protein.

Application Notes

This antibody has been used in  various FACS analyses, for instance, to show that CD27:CD70 and 4-1BB:4-1BBL   interactions are needed for dendritic cell-driven accumulation of antitumor   cytotoxic T lymphocytes following anti-CD40 mAb treatment (French et al,   2007), to examine the underlying mechanisms of the differential T cell   costimulation dependence in viral infections (Welten et al, 2015), and to   explore the therapeutic benefit of using combinations of syngeneic tumor   vaccines that express immune modulators (Manrique-Rincón et al, 2017). This   antibody has been used as a blocking antibody, together with anti-CD70,   anti-CD30L and anti-OX40L antibodies, to evaluate OX40 ligand and CD70 in the   promotion of CD4+ T cell responses (Kurche et al, 2010). Furthermore, this   anti-4-1BBL antibody has been used alongside the anti-CD70, anti-CD27,  anti-CD40 antibodies in a BCL1-bearing mouse model to evaluate the   theurapeutic potency of various combined immmunotherapies against lymphoma   (French et al, 2007).

Antibody First Published

French Ruth et al. Eradication of   lymphoma by CD8 T cells following antiCD40 monoclonal antibody therapy is   critically dependent on CD27 costimulation. Blood. 2007 Jun 1;109(11):4810-5.   PMID:17311995

Note on publication

Describe the generation of this   antibody and its subsequent use alongside with other antibodies to demonstrate that the eradication of lymphoma by CD8 T cells following   anti-CD40 monoclonal antibody therapy is critically dependent on CD27 costimulation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.