Key features and details | |
Cat. No. | MABL-320 |
Name | Anti-5-Hydroxymethylcytosine mAbs |
Clone No. | AFD-AB3/63.3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Dot, IP, IF |
Species Reactivity | Species independent |
Basic Information | |
Specificity | The anti-5-Hydroxymethylcytosine antibody, clone AB3/63.3, detects 5-hydroxymethyl cytosine (hmc, 5hmc), but not 5-methylcytosine or unmethylated cytosine. |
Alternative Name | 5hmc; MABE176; 5hmeC; 5' hydroxymethyl; cytosine; 5'OH methyl cytosine; 5'OHmeC; 5hmc; 5-hmC |
UniProt | |
Immunogen | 5-Hydroxymethylcytosine conjugated to BSA. |
Application Notes | This antibody can be used to detect 5hmC by immunofluorescence and dot blot. Zhang et al. (2014, PMID: 24427299) used the original rat IgG2a format (under the name MABE176) in an immunoprecipitation experiment. |
Antibody First Published | Ficz et al. Dynamic regulation of 5-hydroxymethylcytosine in mouse ES cells and during differentiation. Nature. 2011 May 19;473(7347):398-402 PMID:21460836 |
Note on publication | Describes the generation and use in immunofluorescence of the original antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
