Key features and details | |
Cat. No. | MABL-364 |
Name | Anti-ALCAM mAbs |
Clone No. | AFD- 035-234 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ADCC, FACS |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for ALCAM. ALCAM (Activated leukocyte cell adhesion molecule)” is transmembrane protein, which has a molecular weight of 100 to 105 kDa and is composed of five extracellular Ig-like domains and the intracellular terminus having 32 amino acid, and short transmembrane region. It is an immunoglobulin superfamily molecule including ten N-bonding type sugar chain added sites. ALCAM is one of the adhesive molecules, is present on the activated leukocyte and is identified as a ligand molecule to CD6 molecule (which functions as a signal receptor in T cells). ALCAM also functions as an adhesion factor in homophylic (ALCAM-ALCAM) or heterophylic (ALCAM-CD6) interaction. |
Alternative Name | CD166; CD166 antigen; Activated leukocyte cell adhesion molecule |
UniProt | Q13740 |
Immunogen | The original antibody was isolated by panning a phage display library against ALCAM. |
Application Notes | The antibody exhibited ADCC activity on cancer cells expressing ALCAM, such as CW2 and NCI-H441 cells. The IgG1 format of the antibody could detect ALCAM in different cancer lines (040520IT, CHOK1SV,HepG2, HLF, Caki-1, CCFRC1, ACHN, 293T, PC14, SKOv3, BT474) by FACS (US9388249B2). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
