Key features and details | |
Cat. No. | MABL-365 |
Name | Anti-Alkaline phosphatase mAbs |
Clone No. | AFD- VI-AP (2D5/5B8) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, FC, IF |
Species Reactivity | Cow |
Basic Information | |
Specificity | This antibody recognizes the calf alkaline phosphatase enzyme, and does not cross-react with humans. |
Alternative Name | ALP |
UniProt | P26151 |
Immunogen | The original mouse IgG1 version of this antibody was raised by immunizing mice against calf alkaline phosphatase. |
Application Notes | This original mouse IgG1 antibody mostly gets used as a negative control antibody, as the name suggests. One group used the antibody with immunofluorescence to control for other factors that could provide cell lysis, and provide a better view on how effective their own antibodies were (Sugita et al., 1986; PMID: 3456339). Another group used this antibody as a negative control in an immunofluorescence assay measuring the expression of certain antigens on human T-cells (Pickl et al., 1996; PMID: 8707334). A final group used the antibody with immunocytochemistry and flow cytochemistry as negative control in their research about the oncological proliferation marker Ki-76 (Nies et al., 2018; PMID: 30176186) |
Antibody First Published | Sugita et al. Use of a cocktail of monoclonal antibodies and human complement in selective killing of acute lymphocytic leukemia cells. Int J Cancer. 1986 Mar 15;37(3):351-7 PMID:3456339 |
Note on publication | It is the first mention of the original VI-AP clone, and describes the usuage of this antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
