Key features and details | |
Cat. No. | MABL-397 |
Name | Anti-ALY/REF export factor mAbs |
Clone No. | AFD-Nb395 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human THO complex subunit 4 (ALYREF). This protein is an export adapter involved in nuclear export of spliced and unspliced mRNA. Antibodies against ALYREF/THOC4 are found in sera of patients with systemic lupus erythematosus (SLE). |
Alternative Name | ALYREF; ALY; BEF; THOC4; Tho4; THO complex subunit 4; Ally of AML-1 and LEF-1; Transcriptional coactivator Aly/REF; bZIP-enhancing factor BEF |
UniProt | Q86V81 |
Immunogen | The original antibody was isolated from a VHH library generated by immunization of an alpaca with whole glioblastoma multiforme (GBM) cells. |
Application Notes | This antibody was used for identification of ALYREF protein in cytosolic and membrane protein fractions of glioblastoma (GBM) tissues using western blotting. It was reported that ALYREF expression was high in GBM compared to reference samples. It was reported that CRMP1 was a good marker to distinguish glioma tissues from reference tissues. ELISA was used for the screening of the nanobody library and identification of specific binders that showed specificity for GBM proteins (PMID: 28498803). |
Antibody First Published | Jovčevska et al. Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates. Oncotarget. 2017 Jul 4;8(27):44141-44158.PMID:28498803 |
Note on publication | Describes the isolation of nanobodies against various antigens overexpressed in glioblastoma multiforme. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
