Key features and details | |
Cat. No. | MABL-398 |
Name | Anti-Amiloride mAbs |
Clone No. | AFD- BA7.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | immunoblot, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for the 3,5-diaminopyrazinyl moiety of amiloride. Analogs with a benzyl on the 5-positition of the pyrazine ring or with the subsitution of an iodo for the chloro group in the 6- position of the ring don't bind. An analog with a benzyl substituent on a terminal nitrogen of the guanidinium group has an affinity for the antibody similar to amiloride. |
Alternative Name | |
UniProt | |
Immunogen | The antibody was raised by immunising BALB/c mice with an intraperitoneal injection of an amiloride analogue coupled to BSA. |
Application Notes | The antibody has been characterised using immunoblot analysis (Lin et al. 1994) and ELISA. |
Antibody First Published | Kleyman et al. Distinct epitopes on amiloride Am. J. Physiol. 257 (Cell C1135-C1141, 1989 PMID:2481976 |
Note on publication | Describes the generation of polyclonal and monoclonal antibodies for amiloride |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
